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safe2260330 artist:capnpea562 applejack207514 twilight sparkle370254 alicorn334389 earth pony542741 pony1691842 comic:three apples88 g42120862 behaving like a bird773 black and white18634 bucket3388 comic140067 derp8648 dialogue99654 duo202010 duo female41138 female1900954 frown38383 furry reminder52 grass17126 grayscale51781 mare800521 monochrome180040 mouth hold24937 nest721 nesting instinct34 notepad745 preening1185 prone37956 speech bubble43742 spread wings104823 tent1446 tree54076 twilight sparkle (alicorn)154499 umbrella3761 wide eyes20374 y'all170

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Pea

@lackey_h  
Yeah- the intention is that the strength of her instinct is an anomaly caused by her body suddenly changing (like adult onset bird-puberty). The original script had a couple lines explaining that she would adjust to the instinct over time, but it just felt like an unnecessary justification that took the punch out of the joke.
Chilly Pepper
The End wasn't The End - Found a new home after the great exodus of 2012

Tea Drinker
As a Texan, that second panel gave me a chuckle, though I can understand how Applejack could have done that out of habit.
 
Y’all = You all, plural, … ustedes.
lackey_h
Wallet After Summer Sale -

@BigMax  
Instinct is based on a kind of biological pre-programming, presumably such programming would be part of Twilights new body and - unlike someone who grew up with that programming - she’d have a much harder time ignoring it.
 
A pegasi likely wouldn’t just go and build a nest, or gnaw their wings automatically like Twilight seems to be doing because they’ve either learned not to do that as they grew up or have more modern ways of dealing with the issue. (Though now i imagine baby pegasi have an affinity for finding and/or making snuggly places to hide in, and even adult pegasi would lean towards the more nest-y feeling of sleeping in a fluffy cloud bed).
lackey_h
Wallet After Summer Sale -

Well… I guess a rush of random instincts and such would be expected if your were put into what is, essentially, a whole new body.
 
And most of them could probably seem pretty damn disgusting (see: preening) to someone who never grew up with it as a concept (thus clearly explaining Twis look in the last panel. As long as she’s in her happy place then she was never nomming all the grease, tics and such out of her wings, nope, nosiree, it never happened.)